Multiple shoot induction and regeneration of Vanilla borneensis Rolfe - a critically endangered orchid of Assam, India

In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – critically endangered orchid through multiple shoot regeneration. Through in vitro regeneration from both nodal and tip explants, maximum (100%) induction was observed. The minimum time required bud observed (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 as compared to explants. Maximum explants TDZ (6.82 mM). However, length 15% CW number of nodes (5.27±0.33) per after 90 days. (80-100%) root initiation almost all concentrations NAA. shortest found NAA (5.37 Further, acclimatization period be 15 days 70% while 60% survivability field condition. This efficient method V. could successfully used mass propagation well conservation wild orchid.